| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183639 |
| Chromosome: | chromosome 9 |
| Location: | 6565090 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408051 | (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGTGCCGTCCGGCCCGCCCCGCCGCCG |
| Internal bar code: | AGGGTGCCGCTAATAGGGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 88.1264 |
| LEAP-Seq n confirming: | 16180 |
| LEAP-Seq n nonconfirming: | 2180 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGTTCACTTGGGGTT |
| Suggested primer 2: | TGTTTTGTGTGCGTGAGTGA |