| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183639 |
| Chromosome: | chromosome 9 |
| Location: | 6565095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408051 | (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTCGGTCCCCTAGCGGTCCTGAGTCCT |
| Internal bar code: | CGCCTTCAAGCGGAATAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 93.4908 |
| LEAP-Seq n confirming: | 33724 |
| LEAP-Seq n nonconfirming: | 2348 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGTTCACTTGGGGTT |
| Suggested primer 2: | TGTTTTGTGTGCGTGAGTGA |