| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183646 |
| Chromosome: | chromosome 12 |
| Location: | 5458781 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530600 | GLN3 | Glutamine synthetase; (1 of 2) PTHR20852:SF57 - GLUTAMINE SYNTHETASE 2 CYTOPLASMIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCGCTGCCGCGTGTACACGCACCTGTC |
| Internal bar code: | GTTCGGCCCGGGGGCTAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.0246 |
| LEAP-Seq n confirming: | 2538 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAAGACCGTGCTCAAG |
| Suggested primer 2: | GGTTCTGCTGTCCGCTAGAC |