| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183673 |
| Chromosome: | chromosome 12 |
| Location: | 7814257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553450 | CNG1 | Cyclic-nucleotide gated potassium channel; (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTCCCGCCAAGCGCCTCGCGCCCCCAC |
| Internal bar code: | ATCTCGTCATCTGTTCGTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.6374 |
| LEAP-Seq n confirming: | 6046 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACTTTGTCGTGGAACTT |
| Suggested primer 2: | CTCCTCCTGCTACCACCAAG |