| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183678 |
| Chromosome: | chromosome 17 |
| Location: | 3489015 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATACGCTCGCATTACTATGTCACCTCCT |
| Internal bar code: | TCGCTGTTCTGTGCGACCTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 99.4243 |
| LEAP-Seq n confirming: | 13125 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTTGAGCGAGTAGCTG |
| Suggested primer 2: | CAGACATGGATACCACGCAC |