| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183693 |
| Chromosome: | chromosome 6 |
| Location: | 8691732 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309650 | (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATAGCTTGCCGTGGCACCGCCGCGCGC |
| Internal bar code: | ATGTTGAGGCTCAAATCCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1115 |
| LEAP-Seq percent confirming: | 99.5761 |
| LEAP-Seq n confirming: | 16680 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAAGAAGCCCCGTATTG |
| Suggested primer 2: | GTTGTTCTTCTCAAAGCCGC |