| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183733 |
| Chromosome: | chromosome 12 |
| Location: | 8380124 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549050 | STR1 | Strictosidine synthase; (1 of 1) 4.3.3.2 - Strictosidine synthase / Strictosidine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCGAAGTTGTAGCCAGTGGTATTGGGG |
| Internal bar code: | TTGGGTCCGGGCAGGCTGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 87.7406 |
| LEAP-Seq n confirming: | 3693 |
| LEAP-Seq n nonconfirming: | 516 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGGAGTGGAACACGAGG |
| Suggested primer 2: | TGAATCCCAGCAGGTAGGAC |