| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183763 |
| Chromosome: | chromosome 3 |
| Location: | 6598545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196350 | (1 of 3) K09858 - SEC-C motif domain protein (K09858) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCATGGAGGCGGAGACAGGGCTTGCATC |
| Internal bar code: | TGGGGGGCTTGGTGACTATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.6298 |
| LEAP-Seq n confirming: | 21801 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAGTTGATGAGCCTTG |
| Suggested primer 2: | GTGATGAGGCGACAGAGTGA |