| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183763 |
| Chromosome: | chromosome 9 |
| Location: | 5723098 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401701 | (1 of 1) IPR001104//IPR010721 - 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal // Protein of unknown function DUF1295 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCTGGGATGGCTGTGTGTGGACCTGG |
| Internal bar code: | TGCGGTTGGGGCTACCCCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 94.8197 |
| LEAP-Seq n confirming: | 3734 |
| LEAP-Seq n nonconfirming: | 204 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTGGGTTTGGAGCAAG |
| Suggested primer 2: | GCTGCCTTTGTCATCCTAGC |