| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183771 |
| Chromosome: | chromosome 3 |
| Location: | 7715055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203550 | GT90-3,GT90F3 | GT90 family protein 3; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCCCCTCGCCCTTGCCGCCTTGGGCC |
| Internal bar code: | ACCTCCCTTCACAGTAGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.552 |
| LEAP-Seq n confirming: | 6667 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACCTGGAGGCAATGGT |
| Suggested primer 2: | CATCAGCTCCAAGTTCGACA |