| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183803 |
| Chromosome: | chromosome 10 |
| Location: | 293546 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419900 | 3'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATATCGCTCATCATTCGCGTTCACAAG |
| Internal bar code: | ACAGCTACCGGGGTCGGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 94.6108 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACATGCTAGGTAACCCG |
| Suggested primer 2: | TGGTGCTGTCGAAGCAATAG |