| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183813 |
| Chromosome: | chromosome 13 |
| Location: | 3237580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585750 | FAO11 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF196 - FAD NAD BINDING OXIDOREDUCTASES // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCGAGGCAGATGAACCGTCAGGTGTC |
| Internal bar code: | GGGGCTGACGGACGAACGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 98.7478 |
| LEAP-Seq n confirming: | 2760 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTTGTCAAGTTAGCGG |
| Suggested primer 2: | CGTTCTAGACGCAGAGGACC |