| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183832 |
| Chromosome: | chromosome_3 |
| Location: | 5633472 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g187200 | LC6,DLL2,ODA13 | Flagellar outer arm dynein light chain 6, LC6 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAGTGCTGGAGGCTTGGGCTGCGGCCTTG |
| Internal bar code: | GCGGTCGCATAAATGGGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 99.0905 |
| LEAP-Seq n confirming: | 10023 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGTCAAAGATCAGTGC |
| Suggested primer 2: | TCTACGCGACGAACTGATTG |