| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183833 |
| Chromosome: | chromosome 13 |
| Location: | 3142034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585100 | (1 of 2) IPR002014//IPR008942 - VHS domain // ENTH/VHS | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCGCCGCCTCCAACATCACACCTGCG |
| Internal bar code: | GCCTGAATATTGGCCTAGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 32.3529 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGTACGCGCTTCGTTTT |
| Suggested primer 2: | TGACCTGATCTCACTCGACG |