| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183843 |
| Chromosome: | chromosome 13 |
| Location: | 1303267 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571150 | NUOP1 | NADH:ubiquinone oxidoreductase 10 kDa subunit | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGGCGCCTCATACACGACGTAGCCGCC |
| Internal bar code: | GACGTCGACCTTGCACAATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.8988 |
| LEAP-Seq n confirming: | 1975 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGTGCACATTACCTCCT |
| Suggested primer 2: | TCGCGATGTGAATACTCTGC |