| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183846 |
| Chromosome: | chromosome 8 |
| Location: | 138436 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358538 | (1 of 1) K06443 - lycopene beta-cyclase (lcyB, crtL1, crtY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGATAGAGGTGTTGAATGAAGCAATAC |
| Internal bar code: | GTCAGCGCCGCAATGGGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.8217 |
| LEAP-Seq n confirming: | 5598 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCTAAATGGTCAAGGGG |
| Suggested primer 2: | CTGTCATTGTTACATGCGGG |