| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183893 |
| Chromosome: | chromosome 9 |
| Location: | 4751331 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397216 | (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGTGGCTGAAGGGGCGGCTCAGCACGC |
| Internal bar code: | CCCCTAACAATGTCAGCAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.3796 |
| LEAP-Seq n confirming: | 4485 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGATAGGGGCGCAGAG |
| Suggested primer 2: | TGGATGATGGACGACTCAAA |