| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183909 |
| Chromosome: | chromosome 2 |
| Location: | 3292973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095082 | (1 of 4) PF00956 - Nucleosome assembly protein (NAP) (NAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGAAAGAGTGCGTCTTTGACCTGACG |
| Internal bar code: | GCTGCCAGGGCCCCGACAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.1963 |
| LEAP-Seq n confirming: | 6295 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGCCATTGTGTTACTT |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |