Insertion junction: LMJ.RY0402.183910_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.183910
Chromosome:chromosome 9
Location:5211869
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g399363 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCACCATCCTCGTTGCCGTCGGCTTCGC
Internal bar code:GCGAGTCGTGCATAGGTTGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:771
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAATGTCCATGTCTGTGG
Suggested primer 2:GCGCTACCACAGCACTTCTC