| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183912 |
| Chromosome: | chromosome 6 |
| Location: | 3323968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277350 | HDA13 | RPD3/HDA1 type histone deacetylase; (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTTCAACACACTATCGAGGTGCGCGG |
| Internal bar code: | GCTTTGCTTTCAGAAACTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 82.5175 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAGCAACGTGTCAAGAG |
| Suggested primer 2: | TGTGATGCGGTGTGTATGTG |