| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183931 |
| Chromosome: | chromosome 10 |
| Location: | 4696717 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453500 | RWP15 | (1 of 17) IPR003035 - RWP-RK domain; RWP-RK transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGTACACGGGCTGCCGTACCGTGTGC |
| Internal bar code: | CCGAGTCGACTAATCGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 90.388 |
| LEAP-Seq n confirming: | 2050 |
| LEAP-Seq n nonconfirming: | 218 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGTCGGTGTTACGGCTC |
| Suggested primer 2: | CTCGTCCTCCTCCTCCTTCT |