| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183938 |
| Chromosome: | chromosome 12 |
| Location: | 5949005 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534500 | (1 of 1) PTHR20955//PTHR20955:SF1 - UNCHARACTERIZED // PROTEIN JAGUNAL HOMOLOG 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTATCACTGAAAGCGTGTGCTCCTCTCC |
| Internal bar code: | CCACACTAGTTTCTCGCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 99.646 |
| LEAP-Seq n confirming: | 4222 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAACCAACCTCAGAGTG |
| Suggested primer 2: | GCTCCATCACCACACAACAC |