| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183946 |
| Chromosome: | chromosome 13 |
| Location: | 1743509 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574551 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGGCAGCGTGGGGCTGGGAGAGCTGCA |
| Internal bar code: | CCCACCGGGTCCCGGGATGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.8089 |
| LEAP-Seq n confirming: | 2612 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGATGGGCATACAGGTT |
| Suggested primer 2: | ATCGCCGAGTTGTTACTGCT |