| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183953 |
| Chromosome: | chromosome 1 |
| Location: | 3531130 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022650 | (1 of 1) 3.5.1.6 - Beta-ureidopropionase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAGCACCGCAAGGTGCGCAGCTGTGCG |
| Internal bar code: | CCAAGCGACTACCTTTGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.6468 |
| LEAP-Seq n confirming: | 9309 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTTGTCCTGATACCCAC |
| Suggested primer 2: | TACCGTGTACCAGGGAAAGC |