| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183953 |
| Chromosome: | chromosome 1 |
| Location: | 7852951 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g058886 | (1 of 1) K06944 - uncharacterized protein (K06944) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGTGGAGCGCGGACATTGTATTGCTTA |
| Internal bar code: | CAGCGGTTCTAAGGTTCTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 99.7074 |
| LEAP-Seq n confirming: | 6134 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCATGCATTCGCAAAGTA |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |