| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183965 |
| Chromosome: | chromosome 8 |
| Location: | 4383303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTCCAGCTGCAGTCGCACGTGTGGGT |
| Internal bar code: | ACTGGAGAGTCAACTCGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | AATGCCTGCTACACCTGACC |