| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183970 |
| Chromosome: | chromosome 14 |
| Location: | 679235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGACCTCGCATTGAACCAAGCAACA |
| Internal bar code: | GAATGCAGGAGGCCGCATGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 72.7626 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTGCCAAAAGTTGTTCC |
| Suggested primer 2: | AGTGTCCTCCGGGTAGTCCT |