| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183975 |
| Chromosome: | chromosome 3 |
| Location: | 2240006 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157700 | COX5C | Cytochrome c oxidase 11 kD subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATGGGGTCTATCTCTGCAAGAACACAA |
| Internal bar code: | CCCCAGTACTGTCACCTGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 99.3295 |
| LEAP-Seq n confirming: | 2222 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATCAAGCAGTGACAGAA |
| Suggested primer 2: | GACATGGCCTCAAACAACCT |