Insertion junction: LMJ.RY0402.183981_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.183981
Chromosome:chromosome 6
Location:3698388
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278143 GAE,GAE4 (1 of 1) 5.1.3.6 - UDP-glucuronate 4-epimerase / Uridine diphosphoglucuronate epimerase; UDP-D-glucuronate 4-epimerase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGGAGTGTCAGTTTGTCAGGACATTCTA
Internal bar code:ACGGTTGGTTAACGCACGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1020
LEAP-Seq percent confirming:98.8956
LEAP-Seq n confirming:2776
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGTTTGTGGACGACATT
Suggested primer 2:CTTAGCTCCCGCCTTCTTTT