| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183989 |
| Chromosome: | chromosome 6 |
| Location: | 8226500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305700 | (1 of 14) IPR010998 - Integrase, Lambda-type, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTTTCGTTGAGTGTACAACTTTTGCAT |
| Internal bar code: | ATCAGCGATTAGTGAATAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 97.3727 |
| LEAP-Seq n confirming: | 16307 |
| LEAP-Seq n nonconfirming: | 440 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGGTTCACATCTGCTTT |
| Suggested primer 2: | ACGTGGCTCTCGCAATAAGT |