| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184024 |
| Chromosome: | chromosome 5 |
| Location: | 165929 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234400 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCTGGCGGTGCTGGCGGCGGTGCAGCA |
| Internal bar code: | CGAATTGAATGCGCGTGTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 99.4585 |
| LEAP-Seq n confirming: | 551 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACTGAACAGAGCTGCCA |
| Suggested primer 2: | TACAGCAGTAACCGCACAGC |