Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.184058 |
Chromosome: | chromosome_1 |
Location: | 6680223 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g048000 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGGTAGGTGGGGTTGTCCGGCTCCTCCAG |
Internal bar code: | GGGTAAACTACATATGTAATTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 664 |
LEAP-Seq percent confirming: | 99.5575 |
LEAP-Seq n confirming: | 225 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGGAGAAAGGCACAGCAA |
Suggested primer 2: | TGGAGGTGTCTTCCCTCAAC |