| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184058 |
| Chromosome: | chromosome 1 |
| Location: | 6680223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTAGGTGGGGTTGTCCGGCTCCTCCAG |
| Internal bar code: | GGGTAAACTACATATGTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 99.5575 |
| LEAP-Seq n confirming: | 225 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAGAAAGGCACAGCAA |
| Suggested primer 2: | TGGAGGTGTCTTCCCTCAAC |