| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184060 |
| Chromosome: | chromosome 9 |
| Location: | 2023787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394350 | NUP155 | Nucleoporin 155; (1 of 1) K14312 - nuclear pore complex protein Nup155 (NUP155, NUP170, NUP157) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTGTGGTCGGGGAGGGAGGGCGCAGGC |
| Internal bar code: | TACGGCACGTAGCTAGTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 97.5215 |
| LEAP-Seq n confirming: | 55754 |
| LEAP-Seq n nonconfirming: | 1417 |
| LEAP-Seq n unique pos: | 154 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGGGTCGCAGTAGGAG |
| Suggested primer 2: | TTCCTGATGACGTCTTTCCC |