Insertion junction: LMJ.RY0402.184062_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.184062
Chromosome:chromosome 6
Location:8560681
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g308400 PTN1 (1 of 1) 3.1.3.16//3.1.3.48//3.1.3.67 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase // Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase / Phosphatidylinositol-3,4,5-trisphosphate 3-phosphohydrolase; PTEN PI-3 phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACATGCCAGGGCCCTGCGCCCCCTGTC
Internal bar code:CGCCCGTAACGGGGGTGTTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:99.787
LEAP-Seq n confirming:2342
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGACCTGGACCTCACAT
Suggested primer 2:CAACGGGTCAGCCTCTCTAC