| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184069 |
| Chromosome: | chromosome 10 |
| Location: | 1279427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427050 | MNP1,INDH1 | (1 of 1) PTHR23264//PTHR23264:SF36 - NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED // SUBFAMILY NOT NAMED; Iron-sulfur protein required for NADH dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGTACTGCGGGGACTTGTTGCTGGTAG |
| Internal bar code: | CAGCCGAGACGCGTAGAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 97.1831 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATAGTCATTGCATCCG |
| Suggested primer 2: | GAGCCCTAGAGGCTTGTGTG |