| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184090 |
| Chromosome: | chromosome 2 |
| Location: | 4031050 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097213 | (1 of 1) PTHR19331//PTHR19331:SF322 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGCAGCTCGCAGACTCATCAAAATGCT |
| Internal bar code: | CGGAATACTGCCGGGGTTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 98.1013 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTCCTCTCCACCTGCTG |
| Suggested primer 2: | GTCGACATGTGTGGAAGCAC |