| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184099 |
| Chromosome: | chromosome 12 |
| Location: | 3164639 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499350 | EFH7 | EF-hand Calcium binding protein; (1 of 1) PTHR12294:SF11 - EF-HAND, CALCIUM BINDING MOTIF-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGAAGCTGCTGAAGAAGCTGCTCAAC |
| Internal bar code: | GCCTCGGCCCTCTACCTATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.2392 |
| LEAP-Seq n confirming: | 2348 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGGTCTTAGCAACACAAT |
| Suggested primer 2: | CTTCTCGGCTATTACGCAGG |