Insertion junction: LMJ.RY0402.184137_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.184137
Chromosome:chromosome 7
Location:4416749
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g340850 CYP743A2,CYP19 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGCTGAGGCGAAGCTGCTCGCGGAGA
Internal bar code:ACCTCAGGTAAAATTACAAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:240
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGTGGTGTCTTTTGGC
Suggested primer 2:TCAAGATGCTCAAGACCGTG