| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184138 |
| Chromosome: | chromosome 1 |
| Location: | 4309065 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029100 | (1 of 1) K10733 - GINS complex subunit 2 (GINS2, PSF2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATATGCGTTATGCGTTACATCACCGACA |
| Internal bar code: | AACTCTTGACTGTGTCGTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1034 |
| LEAP-Seq percent confirming: | 97.3304 |
| LEAP-Seq n confirming: | 1349 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTATACCATGGAGGCAGCG |
| Suggested primer 2: | TGAGCTTGGATGTCTGTTGC |