Insertion junction: LMJ.RY0402.184142_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.184142
Chromosome:chromosome 7
Location:6169496
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356150 (1 of 1) 3.4.24.84 - Ste24 endopeptidase / Prenyl protein-specific endoprotease 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGCTGGGATCACATTCACGATTAATCA
Internal bar code:CAGCTGCTAGTTGGGTGCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:99.2028
LEAP-Seq n confirming:4604
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGTGAGAGGTGTGTGTG
Suggested primer 2:CACCCACCACCTTCCTCTAA