| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184166 |
| Chromosome: | chromosome 7 |
| Location: | 4146896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342600 | (1 of 48) IPR011050 - Pectin lyase fold/virulence factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGCTATTTCTCTCAAGGGAAGATTGGG |
| Internal bar code: | TACACGTACCTAGTAGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.5926 |
| LEAP-Seq n confirming: | 3667 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCACAGGCAATAAAGGT |
| Suggested primer 2: | AGTTACTGGGACGTGATGCC |