| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184166 |
| Chromosome: | chromosome 7 |
| Location: | 4146909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342600 | (1 of 48) IPR011050 - Pectin lyase fold/virulence factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACGATCCTGACACCCCTTGTCCTGCG |
| Internal bar code: | TCTCCCTGTGCGGTGCCCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 97.3655 |
| LEAP-Seq n confirming: | 1774 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCACAGGCAATAAAGGT |
| Suggested primer 2: | AGTTACTGGGACGTGATGCC |