| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184190 |
| Chromosome: | chromosome 16 |
| Location: | 7215258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681914 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGGGGACAGAGCAGATGTCTAAACATG |
| Internal bar code: | CTGGGTCGCGCAAGCGGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 95.6719 |
| LEAP-Seq n confirming: | 1901 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGTGCATTGTGCAGAT |
| Suggested primer 2: | CTTCACAGTGGGTGACGTTG |