| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184225 |
| Chromosome: | chromosome 15 |
| Location: | 432275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636300 | CPN2 | Tubulin folding cofactor B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGGCCCATAACTTTGCACCAAACAT |
| Internal bar code: | CTTCTGAGAGACGATCACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 99.5856 |
| LEAP-Seq n confirming: | 2403 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTGTTGACGAACTTGAG |
| Suggested primer 2: | TGTGTGGCAAGAGCTAAACG |