| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184242 |
| Chromosome: | chromosome 5 |
| Location: | 1146313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245701 | FAP104 | Flagellar Associated Protein 104 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACCGAATACTGTTTTCACAAGGCAGG |
| Internal bar code: | CTACAAGGCATCAGCATGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1133 |
| LEAP-Seq percent confirming: | 99.6325 |
| LEAP-Seq n confirming: | 2440 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGAGACCCACCCCTCC |
| Suggested primer 2: | GGCGCTGATAAGAAGTGGAG |