Insertion junction: LMJ.RY0402.184263_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.184263
Chromosome:chromosome 16
Location:1737775
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGAGCGCCACCAGGGCCGCCAACAGGC
Internal bar code:ACGACTTGTCCCGCATGGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1012
LEAP-Seq percent confirming:97.5744
LEAP-Seq n confirming:3379
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCGGCTATTGCTATTTC
Suggested primer 2:TGATGCTGCTAGCCATGAAC