| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184274 |
| Chromosome: | chromosome 6 |
| Location: | 3482660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278099 | (1 of 2) IPR003382 - Flavoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGCTTTGAAGAGTTTGTCAGCGGCCT |
| Internal bar code: | AATATAATCACTGGGATCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.8214 |
| LEAP-Seq n confirming: | 2235 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCTACCAAAACCTTCG |
| Suggested primer 2: | TAACAATGGAGAGTGCTGCG |