| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184298 |
| Chromosome: | chromosome 13 |
| Location: | 2165141 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578100 | NUP205 | (1 of 1) K14310 - nuclear pore complex protein Nup205 (NUP205, NUP192); Nucleoporin 205 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTACCACCGAGCTTATCCTTTGTACTT |
| Internal bar code: | AACTCAGTGCGTGCTAGCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 98.9154 |
| LEAP-Seq n confirming: | 912 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGAAAAGCGGTACAGGA |
| Suggested primer 2: | GAGGAAGCATAGCAACAGCC |