| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184300 |
| Chromosome: | chromosome_12 |
| Location: | 222487 |
| Confidence (%): | 95 |
| Locus disrupted | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g485000 | FAP182 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCGTACATACTCAGCTTTTCTACAAGCC |
| Internal bar code: | ACTGGCCGTTAAGCGGATCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 99.6799 |
| LEAP-Seq n confirming: | 4671 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGACGCTCCCTCACATTC |
| Suggested primer 2: | CCACATTAGCCTCACCGATT |