Insertion junction: LMJ.RY0402.184311_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.184311
Chromosome:chromosome 4
Location:3761902
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTAACCCCCTCGTAACAATTCGTCTCC
Internal bar code:AATACGTGATAGTAGCTCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:99.7429
LEAP-Seq n confirming:388
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTGAACACAACAACCCA
Suggested primer 2:TAGTACATCCCCCAATCCCA